Design expert as a statistical tool for optimization of 5-ASA-loaded biopolymer-based nanoparticles using Box Behnken factorial design

Abstract Background The overall objective was to prepare a highly accurate nanocarrier system of mesalamine for the treatment ulcerative colitis with increased therapeutic efficacy and targeting. In formulation systems, optimization is critical process understanding nanoformulation variables quality aspects. goal present work determine effect independent variables, i.e., concentrations chitosan, carboxymethyl inulin (CMI), drug on response particle size percent entrapment efficiency mesalamine-loaded nanoparticle using Box Behnken design (BBD). correlation between dependent investigated Design Expert generated mathematical equations, contour, surface designs. Result An optimized batch developed ionotropic gel method selected (A: + 1 level, B: 0 C: ? level) nanoparticles had 184.18 nm, zeta potential 26.54 mV, 88.58%. observed responses were remarkably similar predicted values. morphological studies revealed that formulated spherical, results FTIR DSC indicated drug-polymer compatibility. showed less than 5% release in pH 1.2. colonic region (pH 7.4), more 80 % medication released after 24 h. kinetics study Higuchi Korsemeyer-Peppas models R 2 values 0.9426 0.9784 respectively, indicating linearity, as by plots. This result justified sustained behavior formulation. Conclusion chitosan-CMI has been successfully gelation method. this proposed deliver its desired site. likely have small positive high entrapment. It could be stated from BBD can an active way optimizing carrier delivering therapeutics colon.